1YU4
Major Tropism Determinant U1 Variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 86.684, 129.438, 312.649 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.400 - 1.870 |
| R-factor | 0.18586 |
| Rwork | 0.185 |
| R-free | 0.21024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yu0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.989 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 144743 | |
| Completeness [%] | 99.7 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | PEG 4K, MOPS, Magnesium Chloride, pH 6.5, VAPOR DIFFUSION, temperature 298K |
