1YU0
Major Tropism Determinant P1 Variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 1.1055 |
| Spacegroup name | P 63 |
| Unit cell lengths | 92.258, 92.258, 102.231 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.330 - 1.560 |
| R-factor | 0.12843 |
| Rwork | 0.128 |
| R-free | 0.14478 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.229 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.620 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 69212 | |
| Completeness [%] | 98.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG 550 MME, HEPES, calcium chloride, pH 7.5, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG 550 MME, HEPES, calcium chloride, pH 7.5, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG 550 MME, HEPES, calcium chloride, pH 7.5, VAPOR DIFFUSION, temperature 298K |
