1YTV

Maltose-binding protein fusion to a C-terminal fragment of the V1a vasopressin receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-18
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	51.102, 66.557, 115.723
Unit cell angles	90.00, 95.99, 90.00

Refinement procedure

Resolution	34.430 - 1.800
Rwork	0.199
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a7l
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.430	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.076
<I/σ(I)>	16.8
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.2M Ammonium sulfate, 25%(v/v)PEG 400 and 25%(v/v) glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K