1YTT
YB SUBSTITUTED SUBTILISIN FRAGMENT OF MANNOSE BINDING PROTEIN-A (SUB-MBP-A), MAD STRUCTURE AT 110K
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09-16 |
| Detector | FUJI |
| Wavelength(s) | 1.3565, 1.3849, 1.3854, 1.3860 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.508, 72.216, 45.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.20600 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | |
| Rmerge | 0.077 * |
| Redundancy | 0.036 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 * | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | 1.1 (mM) | ||
| 5 | 1 | reservoir | PEG3350 | 15-20 (%) | or PEG8000 |
| 6 | 1 | reservoir | 20 (mM) | ||
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 8 | 1 | reservoir | 10 (mM) | ||
| 9 | 1 | reservoir | 0.02 (%) |
