1YTO
Crystal Structure of Gly19 deletion Mutant of Human Acidic Fibroblast Growth Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-09 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.390, 109.170, 68.980 |
| Unit cell angles | 90.00, 107.19, 90.00 |
Refinement procedure
| Resolution | 49.100 - 2.100 |
| Rwork | 0.198 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.057 | 0.284 |
| Number of reflections | 38023 | |
| <I/σ(I)> | 39.26 | 5.08 |
| Completeness [%] | 91.6 | 80.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | sodium formate, ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
