1YTI

SIV PROTEASE CRYSTALLIZED WITH PEPTIDE PRODUCT

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	C 2 2 21
Unit cell lengths	63.700, 32.500, 97.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.211
Rwork	0.211
R-free	0.29500
RMSD bond length	0.016
RMSD bond angle	27.000 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.200 *
Rmerge	0.053
Number of reflections	4900
Completeness [%]	93.0	83 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium chloride	4-6 (%sat)
2	1	reservoir	sodium cacodylate	100 (mM)