1YTG

SIV PROTEASE CRYSTALLIZED WITH PEPTIDE PRODUCT

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	51.300, 59.100, 62.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.300
R-factor	0.179
Rwork	0.179
R-free	0.28500
RMSD bond length	0.015
RMSD bond angle	27.300 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.300 *
Rmerge	0.070
Number of reflections	8700
Completeness [%]	97.0	97 *
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	42-50 (%sat)
2	1	reservoir	sodium acetate	200 (mM)
3	1	reservoir	dithiothreitol	1 (mM)