1YST

STRUCTURE OF THE PHOTOCHEMICAL REACTION CENTER OF A SPHEROIDENE CONTAINING PURPLE BACTERIUM, RHODOBACTER SPHAEROIDES Y, AT 3 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	143.700, 139.800, 78.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 3.000
R-factor	0.234
Rwork	0.234
RMSD bond length	0.021
RMSD bond angle	24.500 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	3.000 *
Rmerge	0.138 *
Number of reflections	24200
Completeness [%]	80.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8 *	291 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	2 (mg/ml)
2	1	1	PEG4000	12.5-14.5 (%)
3	1	1		0.22 (M)
4	1	1	beta-D-octylglucoside	8 (mg/ml)
5	1	2	Tris-HCl	15 (mM)
6	1	2	EDTA	1 (mM)
7	1	2	sodium azide	0.1 (%)