1YSR
Crystal Structure of ATP binding domain of PrrB from Mycobacterium Tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.887 |
| Spacegroup name | P 65 |
| Unit cell lengths | 61.047, 61.047, 208.429 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.980 - 1.780 |
| R-factor | 0.2026 |
| Rwork | 0.200 |
| R-free | 0.24595 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ys3 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.821 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.054 | 0.862 |
| Number of reflections | 41427 | |
| <I/σ(I)> | 27.6 | 2.5 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | PEG750MME, Magnesium Chloride, Bis Tris Propane, glycerol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
