1YSP
Crystal structure of the C-terminal domain of E. coli transcriptional regulator KdgR.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.540, 31.540, 37.050 |
| Unit cell angles | 90.00, 92.70, 90.00 |
Refinement procedure
| Resolution | 76.700 - 1.800 |
| R-factor | 0.21426 |
| Rwork | 0.212 |
| R-free | 0.25855 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.648 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear (D*TREK (MSC/RIGAKU)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 15893 | |
| Completeness [%] | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | 30% PEG8K, 0.1M NaCacodylate, o.2M (NH4)2SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
