1YSC

2.8 ANGSTROMS STRUCTURE OF YEAST SERINE CARBOXYPEPTIDASE

Experimental procedure

Spacegroup name	P 21 3
Unit cell lengths	111.800, 111.800, 111.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.800
R-factor	0.162
RMSD bond length	0.016 *
RMSD bond angle	2.700 *
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.043 *
Total number of observations	46348 *
Number of reflections	10909 *
Completeness [%]	93.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		0.1 (M)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	citrate/NaOH	20 (mM)
5	1	reservoir	PEG6000	18-24 (%)
6	1	reservoir		0.3 (M)
7	1	reservoir		0.05 (M)
8	1	reservoir	imidazole/NaOH	100 (mM)