1YS6
Crystal structure of the response regulatory protein PrrA from Mycobacterium Tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.222, 46.538, 65.660 |
| Unit cell angles | 100.29, 101.68, 92.52 |
Refinement procedure
| Resolution | 18.630 - 1.770 |
| R-factor | 0.19097 |
| Rwork | 0.188 |
| R-free | 0.24738 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.913 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 1.810 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.048 | 0.574 |
| Number of reflections | 39823 | |
| <I/σ(I)> | 17.6 | 2 |
| Completeness [%] | 98.3 | 72 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | PEG800, Sodium Cacodylate, calcium acetate, Glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
