1YS0

Crystal Structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the disulfide form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	50.197, 71.156, 73.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.700 - 2.000
R-factor	0.2091
Rwork	0.209
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qb0
RMSD bond length	0.008
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	29.700
High resolution limit [Å]	1.850
Number of reflections	21473

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.25		DTT, Tris, Ammonium Sulfate, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 100.0K