1YRY
Crystal structure of human PNP complexed with MESG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.427 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 138.990, 138.990, 159.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pwy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.850 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.790 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 56880 | |
| <I/σ(I)> | 6 | 1.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 290 | Ammonium sulfate, Sodium citrate, pH 5.30, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
