1YRP
Catalytic domain of human ZIP kinase phosphorylated at Thr265
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.560, 60.930, 87.950 |
| Unit cell angles | 90.00, 92.17, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.24508 |
| Rwork | 0.244 |
| R-free | 0.26842 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 10171 | |
| <I/σ(I)> | 11.3 | 3.7 |
| Completeness [%] | 97.2 | 95.5 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | PEG, MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
