1YRN
CRYSTAL STRUCTURE OF THE MATA1/MATALPHA2 HOMEODOMAIN HETERODIMER BOUND TO DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 94 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-07-23 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 132.380, 132.380, 45.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.29800 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.930 |
| Data reduction software | R-AXIS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.055 |
| Total number of observations | 76841 * |
| Number of reflections | 18106 |
| Completeness [%] | 94.0 |
| Redundancy | 4.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 100 (mM) | precipitant |
| 2 | 1 | drop | 20 (mM) | precipitant | |
| 3 | 1 | drop | cobaltic hexamine | 5-10 (mM) | precipitant |
| 4 | 1 | reservoir | HEPES | 150-200 (mM) | |
| 5 | 1 | reservoir | 20 (mM) |
