1YRH
Crystal Structure Of Trp Repressor Binding Protein Wrba in complex with FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | .9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 122.327, 122.327, 208.735 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.110 |
| R-factor | 0.19954 |
| Rwork | 0.198 |
| R-free | 0.23320 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.159 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.170 | 0.320 |
| Number of reflections | 32107 | |
| <I/σ(I)> | 5.6 | 4.5 |
| Completeness [%] | 97 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 293 | 2.6M AMSULFATE, 20% GLYCEROL, pH 4.5, VAPOR DIFFUSION, temperature 293K |
