1YR9
PAB0955 crystal structure : a GTPase in GDP and PO4 bound form from Pyrococcus abyssi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.820, 84.051, 53.167 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.232 |
| Rwork | 0.225 |
| R-free | 0.34600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yr6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.175 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.093 | 0.455 |
| Number of reflections | 6729 | |
| <I/σ(I)> | 15.19 | 4.6 |
| Completeness [%] | 97.8 | 93.8 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 8% PEG6000, 1M Lithium chloride, 50mM NaCl, 10% glycerol, 0.1M Citric Acid , 1mM GTP, 20mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
