1YR8

PAB0955 crystal structure : a GTPase in GTP bound form from Pyrococcus abyssi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-26
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 32 2 1
Unit cell lengths	60.710, 60.710, 116.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.24201
Rwork	0.236
R-free	0.32500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yr6
RMSD bond length	0.009
RMSD bond angle	1.192
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.075	0.400
Number of reflections	10088
<I/σ(I)>	20.76	7.03
Completeness [%]	98.5	98.2
Redundancy	11.6	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	12% peg4000, 0.1M ammonium acetate, 0.1M tri-sodium citrate dihydrate, 20mM DTT, 10mM EDTA, 0.65mM GTP, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K