1YQV

The crystal structure of the antibody Fab HyHEL5 complex with lysozyme at 1.7A resolution

Replaces:  3HFLReplaces:  2HFL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	54.459, 74.258, 78.256
Unit cell angles	90.00, 101.65, 90.00

Refinement procedure

Resolution	30.000 - 1.700
Rwork	0.195
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3HFL
RMSD bond length	0.005
RMSD bond angle	1.290
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.700
Number of reflections	62951
<I/σ(I)>	16.3
Completeness [%]	94.0
Redundancy	1.86

Crystallization Conditions

crystal ID	method	pH	temperature	details
1