1YQD

Sinapyl Alcohol Dehydrogenase complexed with NADP+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	298
Detector technology	CCD
Collection date	2002-04-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.2822
Spacegroup name	P 21 21 2
Unit cell lengths	76.477, 137.796, 69.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.320 - 1.650
R-factor	0.195
Rwork	0.195
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unrefined structure of the same protein solved by MAD
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.300	1.700
High resolution limit [Å]	1.650	1.650
Number of reflections	83917
Completeness [%]	94.1	89.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	polyethylene glycol monomethyl ether 5000, polyethylene glycol 400, MOPSO, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K