1YPV
Structure of human thymidylate synthase at low salt conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-03 |
| Detector | SBC-1 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 95.772, 95.772, 81.851 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.330 - 1.800 |
| R-factor | 0.2265 |
| Rwork | 0.227 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.563 |
| Number of reflections | 40526 | |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 93.6 | 91.2 |
| Redundancy | 4.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, TRIS BUFFER, AMMONIUM SULFATE, 2-MERCAPTOETHANOL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
