1YP5
Yeast Myo5 SH3 domain, tetragonal crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 0.81 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 34.620, 34.620, 99.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.680 |
R-factor | 0.19156 |
Rwork | 0.190 |
R-free | 0.22235 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.162 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.750 |
High resolution limit [Å] | 1.680 | 1.680 |
Number of reflections | 7261 | |
<I/σ(I)> | 17.6 | 3.7 |
Completeness [%] | 96.8 | 99.6 |
Redundancy | 5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | sodium malonate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |