1YOO
ASPARTATE AMINOTRANSFERASE MUTANT ATB17 WITH ISOVALERIC ACID
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-08 |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 157.000, 84.840, 78.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.187 * |
| Rwork | 0.162 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1art |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.600 * |
| Data reduction software | PROCESS ((RIGAKU)) |
| Data scaling software | PROCESS ((RIGAKU)) |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 2.800 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.056 | 0.120 |
| Total number of observations | 61169 * | |
| Number of reflections | 17348 | |
| <I/σ(I)> | 19.8 | 3.2 |
| Completeness [%] | 98.6 | 98.3 |
| Redundancy | 3.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | drop | sodium isovalerate | 0.2 (M) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 4 | 1 | reservoir | Na-HEPES | 0.1 (M) |
