1YOJ
Crystal structure of Src kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00075 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.867, 62.542, 73.902 |
| Unit cell angles | 100.52, 88.88, 89.99 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.2162 |
| Rwork | 0.214 |
| R-free | 0.25811 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | active Src kinase domain in complex with CGP77675 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.496 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.135 | |
| Number of reflections | 51365 | |
| <I/σ(I)> | 6.8 | 3.7 |
| Completeness [%] | 93.9 | 90.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 20% ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
