1YMY
Crystal Structure of the N-Acetylglucosamine-6-phosphate deacetylase from Escherichia coli K12
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97934, 0.97911, 0.97166 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.226, 114.023, 80.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.600 |
| R-factor | 0.219 |
| Rwork | 0.212 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 23035 | |
| Completeness [%] | 96.8 | 91.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Ammonium phosphate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
