1YMU

SIGNAL TRANSDUCTION PROTEIN CHEY MUTANT WITH MET 17 REPLACED BY GLY (M17G)

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1993-07-27
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 31
Unit cell lengths	53.400, 53.400, 92.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.198
Rwork	0.198
RMSD bond length	0.013
RMSD bond angle	1.690
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300	2.300 *
Rmerge	0.069
Total number of observations	33574 *
Number of reflections	10135
Completeness [%]	77.6	73.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2	20 *	pH 7.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)
3	1	reservoir	ammonium sulfate	3 (M)
4	1	reservoir	Tris-HCl	0.1 (M)