1YMP

The Crystal Structure of a Partial Mouse Notch-1 Ankyrin Domain: Repeats 4 Through 7 Preserve an Ankyrin Fold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	98
Wavelength(s)	0.9
Spacegroup name	P 31
Unit cell lengths	75.789, 75.789, 45.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.880 - 2.200
Rwork	0.206
R-free	0.30900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n0r
RMSD bond length	0.011
RMSD bond angle	1.690
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.043
Number of reflections	14979
Completeness [%]	85.6	85.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG 10000, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K