1YLX
Crystal Structure of a Protein of Unknown Function from Bacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.189, 70.156, 81.361 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.680 - 1.600 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Phasing software | HKL-3000 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.079 | |
| Number of reflections | 49668 | |
| Completeness [%] | 94.5 | |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MPD, calcium chloride, bis-tris pH 6.5, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
