1YLV
SCHIFF-BASE COMPLEX OF YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE WITH LAEVULINIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-10 |
| Detector | PRINCETON 2K |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 103.400, 103.400, 168.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.150 |
| Rwork | 0.207 |
| R-free | 0.26500 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1aw5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.027 |
| Data reduction software | XDS |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | CCP4 |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.600 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.063 | 0.135 |
| Number of reflections | 23348 | |
| <I/σ(I)> | 8.2 | 6 |
| Completeness [%] | 96.8 | 77.7 |
| Redundancy | 13.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | AS FOR 1AW5 WITH 10MM LAEVULINIC ACID, pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 2 (mg/ml) | |
| 2 | 1 | drop | laevulinic acid | 10 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 0.2 (M) | |
| 4 | 1 | reservoir | PEG6000 | 50 (%(w/v)) | |
| 5 | 1 | reservoir | Zn2+/mercaptoethanol |
