1YLN
The Crystal Structure of the Protein of Unknown Function VCA0042 from Vibrio cholerae O1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-25 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.429, 56.429, 171.128 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.190 - 2.200 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.142 | 0.459 |
| Number of reflections | 30326 | |
| <I/σ(I)> | 14.83 | 2.04 |
| Completeness [%] | 99.5 | 95.7 |
| Redundancy | 9.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 1.0 M K/Na tartrate, 0.1M Tris, 0.2M Li2SO4, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
