1YLM
Structure of Cytosolic Protein of Unknown Function YutE from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2004-11-14 |
| Detector | SBC-1 |
| Wavelength(s) | .97940 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.914, 72.081, 76.552 |
| Unit cell angles | 90.00, 115.24, 90.00 |
Refinement procedure
| Resolution | 33.600 - 1.830 |
| R-factor | 0.20165 |
| Rwork | 0.200 |
| R-free | 0.23850 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.318 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.600 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 30664 | |
| Completeness [%] | 89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | .075 Tris, 1.5M Ammonium sulfate, 25% glycerol, pH 8.5, VAPOR DIFFUSION, temperature 298K |
