1YLF
X-ray crystal structure of BC1842 protein from Bacillus cereus, a member of the Rrf2 family of putative transcription regulators.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-22 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.080, 73.080, 157.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| R-factor | 0.216 |
| Rwork | 0.217 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.101 | 0.517 |
| Number of reflections | 17282 | |
| <I/σ(I)> | 25.2 | 2.02 |
| Completeness [%] | 98.3 | 83.6 |
| Redundancy | 17 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.2 M Na Cl, 25% PEG 3350, Tris_Cl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
