1YL7
the crystal structure of Mycobacterium tuberculosis dihydrodipicolinate reductase (Rv2773c) in complex with NADH (crystal form C)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8042 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 238.600, 67.520, 154.490 |
| Unit cell angles | 90.00, 117.08, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.340 |
| R-factor | 0.18307 |
| Rwork | 0.181 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1P9L (MONOMER) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.384 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.380 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.091 | 0.301 |
| Number of reflections | 80382 | |
| <I/σ(I)> | 18.7 | 3.3 |
| Completeness [%] | 86.6 | 76.6 |
| Redundancy | 18.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 100 mM Tris/HCl, 26 % PEG 3350, 140 mM MgCl2 + 3 mM NADH in the protein solution, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
