1YKY
Effect of alcohols on protein hydration
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.294, 79.294, 37.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.420 - 1.900 |
Rwork | 0.185 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2lym |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.420 | 2.030 |
High resolution limit [Å] | 1.900 | 1.940 |
Rmerge | 0.062 | |
Number of reflections | 9935 | |
Redundancy | 1 | 14.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | sodium chloride and sodium acetate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |