1YKD
Crystal Structure of the Tandem GAF Domains from a Cyanobacterial Adenylyl Cyclase: Novel Modes of Ligand-Binding and Dimerization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.958, 66.265, 70.212 |
| Unit cell angles | 103.57, 96.56, 115.15 |
Refinement procedure
| Resolution | 48.200 - 1.900 |
| R-factor | 0.18401 |
| Rwork | 0.182 |
| R-free | 0.21532 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.069 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.200 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 74903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 295 | ammonium sulfate, bis-tris propane, glycerol, Tris, MgCl2, betamercaptoethanol, 3',5' cyclic adenosine monophosphate, pH 8.8, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
