1YK5
Pyrococcus abyssi rubredoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 286 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.301, 59.513, 80.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.370 - 1.790 |
| R-factor | 0.158 |
| Rwork | 0.158 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1brf |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.712 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.370 | 1.810 |
| High resolution limit [Å] | 1.790 | 1.780 |
| Number of reflections | 24841 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 98.1 | 89 |
| Redundancy | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | Ammonium sulfate, MES, Dioxane, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
