1YJZ

K226M Mutant Of Serine Hydroxymethyltransferase From B. Stearothermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-10-10
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	61.048, 106.524, 56.903
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.21477
Rwork	0.212
R-free	0.26084
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kkj
RMSD bond length	0.013
RMSD bond angle	0.036
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge		0.084
Number of reflections	22373
<I/σ(I)>	25.1
Completeness [%]	84.6	89.1
Redundancy	5.7	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Hepes, MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K