1YJL
Reduced Peptidylglycine alpha-Hydroxylating Monooxygenase in a new crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.764, 66.019, 69.526 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.670 - 2.400 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1opm |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.927 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4_3.2.5) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.940 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.065 | 0.445 |
| Total number of observations | 7310 | |
| Number of reflections | 11174 | |
| <I/σ(I)> | 4.3 | 1.7 |
| Completeness [%] | 99.5 | 99.68 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 4000, Magnesium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
