1YJ0
Crystal Structures of Chicken Annexin V in Complex with Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2002-03-09 |
| Wavelength(s) | 0.9700 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 106.593, 106.593, 106.593 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.520 - 2.950 |
| R-factor | 0.2111 |
| Rwork | 0.211 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.520 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.120 | 0.408 |
| Number of reflections | 9102 | |
| <I/σ(I)> | 21.6 | |
| Completeness [%] | 99.3 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | Ammonium Sulfate, zinc chloride, HEPES, pH 8.0, VAPOR DIFFUSION, temperature 298K |
