1YIS
Structural genomics of Caenorhabditis elegans: adenylosuccinate lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97928 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 124.242, 124.242, 165.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| Rwork | 0.174 |
| R-free | 0.20170 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 48307 | |
| <I/σ(I)> | 13.9 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 1.49 M AMMONIUM SULFATE, 0.1 M CITRIC ACID, PH 5.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295.0K |
