1YIO
Crystallographic structure of response regulator StyR from Pseudomonas fluorescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.196, 35.679, 73.877 |
| Unit cell angles | 90.00, 99.15, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.20454 |
| Rwork | 0.201 |
| R-free | 0.27455 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.521 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.085 | 0.424 |
| Number of reflections | 10478 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 99.3 | 90.98 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 277 | PEG 4000, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
