1YID
Crystal structure of tryptophanyl tRNA synthetase II from Deinococcus radiodurans in complex with ATP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2004-08-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 213.560, 58.680, 85.700 |
| Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.26 |
| Rwork | 0.260 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 20856 | |
| Completeness [%] | 80.0 | 80 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG4K, 0.2M DiAMMONIUM HYDROGEN PHOSPHATE, 1mM ATP, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
