1YI9
Crystal Structure Analysis of the oxidized form of the M314I mutant of Peptidylglycine alpha-Hydroxylating Monooxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.479, 66.458, 70.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.200 - 1.700 |
| R-factor | 0.20239 |
| Rwork | 0.201 |
| R-free | 0.23261 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1opm |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.182 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.041 | 0.375 |
| Number of reflections | 28704 | |
| <I/σ(I)> | 8.8 | 1.8 |
| Completeness [%] | 87.6 | 48.8 |
| Redundancy | 2.5 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 4000, magnesium chloride, Tris , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
