1YH2
Ubiquitin-Conjugating Enzyme HSPC150
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 54.831, 54.831, 183.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.20372 |
| Rwork | 0.202 |
| R-free | 0.23793 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jbb |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.664 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.036 | 0.266 |
| Number of reflections | 19774 | |
| <I/σ(I)> | 44.8 | |
| Completeness [%] | 99.5 | 98 |
| Redundancy | 5.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 4M Sodium Formate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
