1YGY

Crystal Structure of D-3-Phosphoglycerate dehydrogenase From Mycobacterium tuberculosis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Detector technology	CCD
Collection date	2003-12-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97937, 0.97961
Spacegroup name	P 65 2 2
Unit cell lengths	165.513, 165.513, 218.144
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.800 - 2.300
R-factor	0.20715
Rwork	0.205
R-free	0.24853
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.478
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.095	0.404
Number of reflections	78223
<I/σ(I)>	24.7	4.8
Completeness [%]	99.7	99.9
Redundancy	14.3	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	sodium potassium tartrate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	sodium potassium tartrate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K