1YGH
HAT DOMAIN OF GCN5 FROM SACCHAROMYCES CEREVISIAE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.040, 66.510, 80.190 |
| Unit cell angles | 90.00, 97.71, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TETRAHYMENA THERMOPHILA GCN5 HAT DOMAIN |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.250 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.030 * | |
| Total number of observations | 181066 * | |
| Number of reflections | 31832 | |
| <I/σ(I)> | 23.6 | 13 |
| Completeness [%] | 96.4 | 98 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 4 * | 400 MM AMMONIUM SULFATE, 20% - 25% PEG 8000, pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 400 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 20-25 (%) |
