1YGB

Crystal Structure of the catalytic fragment of alanyl-tRNA synthetase in complex with L-serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	200
Detector technology	CCD
Collection date	2004-07-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97976
Spacegroup name	P 41 21 2
Unit cell lengths	74.091, 74.091, 173.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.480
R-factor	0.21
Rwork	0.199
R-free	0.23200
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1riq
RMSD bond length	0.018
RMSD bond angle	1.257
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.480
Number of reflections	17674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	298	PEG 5000 mme, NaCl, Tris-Cl, Tris-base, L-serine , pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K