1YG9
The structure of mutant (N93Q) of bla g 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.600, 38.600, 71.500 |
| Unit cell angles | 90.00, 100.90, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.300 |
| Rwork | 0.180 |
| R-free | 0.21250 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 94652 | |
| <I/σ(I)> | 17.4 | 1.7 |
| Completeness [%] | 98.1 | 91.9 |
| Redundancy | 2.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 20% PEG8k, 10mM DTT and 0.2M MgAcetate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
