1YG8
The structure of a V6A variant of ClpP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 99 |
Detector technology | CCD |
Detector | BRANDEIS - B4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.800, 161.000, 186.600 |
Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.225 |
Rwork | 0.220 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | RES 1-20 1YG6 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 147121 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | MPD, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |